Researchers from Fujita Health University School of Medicine in Japan have been investigating COVID-19 to lead to therapeutic options against the severe form of the condition.
Levels of proinflammatory cytokines are dramatically elevated in severe COVID-19 patients, the molecular mechanisms of which remain unclear.
In a recent article in mBio, the researchers elucidated the role of different molecular players involved in the activation of NF-κB signaling, a key mediator in the cytokine pathway.
Most people infected with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus have mild symptoms, but for others, COVID may cause severe pneumonia, acute respiratory distress syndrome, and organ damage. Typically, the symptoms are triggered by an extreme immune response that causes elevated levels of cytokines, or a “cytokine storm.” However, the detailed mechanisms of proinflammatory cytokine production are not fully understood.
Previous studies reported the NF-κB family of transcription factors is an integral component of the process. This makes NF-κB signaling an important COVID-related therapeutic target. Viral nucleocapsid (N) proteins can also interact with transforming growth factor b-activated kinase 1 (TAK1) and IκB kinase (IKK) complexes to activate the NF-κB pathway. Although there has been speculation on defining these interactions, scientists are still trying to determine which viral genes they primarily involve.
To disentangle these mechanisms, Takayuki Murata and Hironiri Nishitisuji from the Fujita Health University School of Medicine, and Kunitada Shimotohno from the National Centre for Global Health and Medicine, Japan, screened 22 SARS-CoV-2 proteins to identify the ones that could activate NF-κB signaling.
Murata said, “We tried to identify the modifiers of cell signals, especially those of inflammatory signals, because inflammation is central to the symptoms of COVID-19.”
The team found two central SARS-CoV-2 proteins—nonstructural protein 6 (NSP6) and open reading frame 7a (ORF7a)—were essential for activating the NF-κB pathway. Consistent with their hypothesis, the expression of NSP6 and ORF7a increased the levels of proinflammatory cytokines such as interleukin 8 (IL-8) and interferon gamma-induced protein 10 (IP-10).
The scientists said this suggests the viral components may be activating NF-κB to cause the cytokine storm in the later stages of the infection, since infected individuals experience extreme symptoms during severe COVID-19.
CRISPR
Using CRISPR-Cas9 knockout studies, the team further discovered that NSP6 and ORF7a act via transforming growth factor b-activated kinase 1 (TAK1) and NF-κB essential modulator (NEMO), which are crucial players in the NF-κB pathway. The knockdown of TAK1/NEMO significantly reduced SARS-CoV-2-induced NF-κB activation, indicating these proteins could be considered as potential targets for COVID-19 treatment.
The researchers then looked into the ubiquitination of NSP6 and ORF7a, which could be critical for exerting their downstream effects. Murata and his team found tripartite motif-containing 13 (TRIM 13) and ring finger protein 121 (RNF121) were required for the ubiquitination of NSP6 and ORF7a, respectively. This process seemed to be essential for recruiting NEMO to the NSP6-TAK1 complex and activating NF-κB.
Since the suppression of cytokine production and SARS-CoV-2 replication are both halted by blocking NF-κB signaling, it is possible the virus hijacks the pathway for its replication. “We propose that NF-κB suppression of proinflammatory cytokine responses while maintaining adequate immunity for viral clearance may be a good strategy to stop the virus,” Murata said.
The team said the study may add to the tool box of existing approaches and treatment ideas for COVID-19.
“Our findings offer a better understanding of SARS-CoV-2 pathogenesis and host immune response to the infection,” Murata said.
“Inhibitors of the molecules that mediate NF-κB activation may be used to reduce the severity of COVID-19 symptoms.”